Journal: Molecular Medicine
Article Title: Overexpression of c-Myc triggers p62 aggregation-mediated mitochondrial mitophagy in cabozantinib resistance of hepatocellular carcinoma
doi: 10.1186/s10020-025-01263-w
Figure Lengend Snippet: High Expression of c-Myc Promotes Cabozantinib Resistance in Liver Cancer Cells. A Differential expression pathway heatmap of cells with or without cabozantinib resistance ( GSE174770 ). B Detection of c-Myc expression in subcutaneous tumor models established with resistant and sensitive liver cancer cell lines. C Cell viability assay using CCK-8 method after overexpressing c-Myc in cabozantinib-sensitive cells and knocking down c-Myc in resistant cells. D Western blot analysis of BAX, BCL2, CYTC, Cleaved caspase9 and Cleaved caspase3. E-F Apoptosis detection using flow cytometry after overexpressing c-Myc in cabozantinib-sensitive cells and knocking down c-Myc in resistant cells. F Dose-response analysis of cells after overexpressing c-Myc in cabozantinib-sensitive cells and knocking down c-Myc in resistant cells. The IC50 of SNU-368-S and SNU-739-S increased from 7.25 µM and 6.23 µM to 12.49 µM and 10.60 µM, respectively, while the IC50 of SNU-368-R and SNU-739-R decreased from 17.66 µM and 14.84 µM to 10.52 µM and 9.14 µM, respectively. G Caspase 3/7 Activity Apoptosis Assay to detect caspase3 activity in cells after overexpressing c-Myc in cabozantinib-sensitive cells and knocking down c-Myc in resistant cells. H Construction of subcutaneous tumor models in mice and administration schedule. I-J Images of subcutaneous tumor and changes in tumor volume. K Construction of c-Myc-induced orthotopic liver cancer model using the Sleeping Beauty system, L Gross images of the c-Myc-induced in situ liver cancer mouse model and its appearance after cabozantinib treatment. M-O Statistical analysis of mouse mortality rate, liver/body ratio and tumor number after cabozantinib treatment
Article Snippet: Cleaved caspase9 , CST (#9509) , 1:1000.
Techniques: Expressing, Quantitative Proteomics, Viability Assay, CCK-8 Assay, Western Blot, Flow Cytometry, Activity Assay, Apoptosis Assay, In Situ
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